RNA Aptamer Discovery: SELEX Methods and Emerging Strategies

Review Article

Authors

  • Andrew Michael Reynolds Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, United States Author
  • Xiaohong Tan Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, United States Author

Keywords:

SELEX, Aptamer, Ligand, RNA, Post-selection

Abstract

RNA SELEX is an attractive strategy to develop aptamers for probing and treating disease. This systematic evolution of RNA molecules is a complex process with a variety of modifications available depending on the target or application. The RNA SELEX process is described here with a review of various selection methods. Process design principles, post-selection aptamer discovery pathways, and roadblocks to RNA aptamer development are discussed. This review finishes with recent applications and future directions of RNA aptamer technology.

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Published

2026-08-11

Issue

Vol. 2 No. 2 (2025): RNA Nanomed Volume 2 Issue 2 Year 2025

Section

Articles

License

Copyright (c) 2026 Andrew Michael Reynolds, Xiaohong Tan (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.